Effects of Antifungal Agents for Fungi and Tumor Cells

Effects of Antifungal Agents for Fungi and Tumor Cells Literary Summary of Effects of Antifungal Agents and ÃŽÂ ³ Interferon on Macrophage Cytotoxicity for Fungi and Tumor Cells. The experimenters in this journal describe the influence of antifungal agents on acquisition of the activated state of the microphage. Stating that the macrophages modify their activity in response to the microbes in an infection. The experimenters continue to state that metabolic functions are factors that may affect the way the cells change their state of activation when testing the toxicity of the chemical substance on cultures. The experimenters noted a particular factor, calling it a marker that targets the neoplastic or microbial cells and kills them. The experimenters discovered when using bacillus Calmette-Guerin (BCG); that the peritoneal cells when introduced with limited quantities of endotoxin become fully cytotoxic for susceptible tumor cell lines (Perfect, J. et al., 1987). The experimenters exclaim that it is this tumoricidal activity that is the designated marker for the activated macrophages. Continuing this line of thought the experimenters then state that the 1st signal in this activation process is ÃŽÂ ³ Interferon (IFN- ÃŽÂ ³) when testing the toxicity of the chemical substance on cultures for intracellular infection. Experimenters posed that one hypothesis could be that the antimicrobial agents they were going to use may act against the invading fungi by promoting the host immune response. With that hypothesis; the question the authors were trying to answer in this journal is the study of the effector systems of activated murine macrophages against fungi (Perfect, J. et al., 1987). In this journal the experimenters state that they will be working with three target cells. Murine fibro sarcoma cells (3T12); Cryptococcus neoformans H99/C3D, a clone from a human pathogenic isolate that does not increase capsule size in response to physiological concentrations of carbon dioxide [24]; and Candida parapsilosis, a nonpathogenic strain isolated from the laboratory environment (Perfect, J. et al., 1987). The experimenters in this journal used various research items and obtained supplies from Wilmington Massachusetts, the Trudeau Institute in Saranac Lake New York, Detroit Michigan, Gibco in Grand Island New York, Corning New York, and Salt Lake City Utah. The experimenters performed the laboratory experiments at the Duke University Medical Center in Durham, North Carolina. Having all the various supplies and research items necessary to perform the experiment the experimenters conducted at least three different experiments for each additive. Periodically all the additives, medium and plastics were checked for endotoxin contamination by amebocyte lysate assay (Perfect, J. et al., 1987). C. neoformans or C. parapsilosis (yeasts) were grown overnight and suspended in modified DMEM and adjustments were made by the hemocytometer and counts yielded 103 yeast for a total volume of 0.2 mL per well. Macrophage, Fungistatic, and the antifungal agent assays were washed five times with DMEM before any yeasts were added. As a control, wells without cells were included for each additive. Wells were then cultured after being prepared on Sabourauds agar after lysing of host cells with a chemical compound of deoxycholate at 0.5% (Perfect, J. et al., 1987). The experimenters did a one-way analysis of variance on each set of three of the experiments. The experimenters in case of finding a difference between grounds a multiple comparison analysis by Tukeys method would be used. Visual results were good, having showed correlation with those found using the more quantitative thymidine release assay for tumoricidal activity (Perfect, J. et al., 1987). According to the results, the macrophage activation for tumor killing appeared to work whereas the antifungal agents had no effect. The experimenters found the serum to be with in tolerance range for human therapeutic purposes. The experimenters explain that a significant cytosidal effect by the macrophages on the tumor cell growth was found and that the next step would be to determine whether macrophage activation for tumor cell cytotoxicity correlated with the ability to inhibit or kill fungal cells (Perfect, J. et al., 1987). With previous knowledge and experience in macrophage activation, the experimenters knew that more consistent results could be obtained if the culture medium was to be left throughout the testing. With previous knowledge of this, endotoxin was used because the experimenters knew it would have no direct effect on antifungal activity. The experimenters determined in previous experiments that the azole compounds used had no prior effect. However, results showed dramatic effects on yeast growth. The experimenters postulated that direct antifungal activity was due in part by human error in the preparation and cleansing phase. This meant that a drug must have remained in the macrophage cultures to give those results. Further testing showed active drug remains within the monolayers or the surfaces of the plastic culture vessel despite extensive washing (Perfect, J. et al., 1987). The experimenters removed the cells from the tissue culture container, washed and lysed in 0.5% deoxycholate again assuring no further contaminates. The process was repeated, after 24 hours desired results showed. The experimenters were able to confirm that the activating effect of AMB in tumor cell killing by macrophages (Perfect, J. et al., 1987). The experimenters were able to show that the primed macrophage was made cytotoxic for tumor cells in the presence of therapeutic concentrations of AMB (Perfect, J. et al., 1987). Having acceptable results and demonstrating findings the experimenters had shown that fungicidal activity did stay within the cells even after having been removed from by an antifungal medium. Tests had shown that the compound was biologically active and attached to the cells. The experimenters explain that this may be useful in understanding macrophage-yeast interactions during antifungal treatment (Perfect, J. et al., 1987). Reference Cited Perfect, J., Granger, D., Durack, D. (1987). Effects of Antifungal Agents and ÃŽÂ ³ Interferon on Macrophage Cytotoxicity for Fungi and Tumor Cells. The Journal of Infectious Diseases, 156(2), 316-323. Retrieved from http://www.jstor.org/stable/30136160

Boston Tea Party, Tea Essay

Attention Getter: You probably wouldn’t believe me if I told you that a drug that creates the same signaling activity in the brain as cocaine is sold legally on almost every street corner across the nation. This is a fact however, and the drug is caffeine. It is one of the main ingredients in coffee, and is currently the most frequently used recreational drug in the world. Establish Credibility: I personally consider myself a coffee expert, and have been drinking at least one cup of coffee almost every morning for the last three years. Relate to the audience: Now, I know not every one of you drinks coffee but as you  can tell by the multiple Dutch Bros, Starbucks, and other coffee shops around town it is a very big part of our culture today. Preview: Throughout the course of this speech, I will discuss three major points relating to coffee. First, I will give some information on the history of coffee and when it first came into human culture, followed by the positive health benefits and some of the negative health risks associated with coffee. [First of all, I’m going to talk a little bit about the historical side of coffee and how it first got popular in our culture. ] Body I. Main Point: To understand why coffee is such a big part of modern day society, we must first understand where it originated. A. Sub-point: Coffee was first cultivated on the Arabian Peninsula in the 15th century. 1. Supporting details: Not only were the Arab’s the first to cultivate coffee, but they also were the first to trade coffee. 2. By the 16th century, the popularity of coffee was already expanding and Persia, Egypt, Syria and Turkey all had discovered about the amazing beverage. Sub-point: By the 17th century coffee was still gaining notoriety around  the world, however it was still not a household favorite as it as today. 1. Supporting Details: Coffee houses started sprouting up everywhere in the 1700’s, however tea was still the most popular drink around. 2. In 1773 a heavy tax on tea was inflicted by King George, which led to a pretty famous revolt called The Boston Tea Party. 3. Since people couldn’t afford tea after the tax, coffee quickly took over as the most popular morning beverage across the world, and remains the most popular today. Although coffee has been drank for hundreds of years, the majority of its health  benefits have only recently been discovered in the last few decades. ] II. Main Point: The majority of people drink coffee just to get through each day, however it does have many positive benefits to your health. A. Sub-point: Within just the last few years alone there has been research done showing that coffee may protect against type 2 diabetes, liver cancer, and Alzheimer’s disease. 1. Supporting details: According the Centers for Disease Control and Prevention, liver cancer is the ninth leading cause of cancer and coffee has been shown to reduce risk of liver cancer by 40%. 2. Drinking coffee reduces the risk of diabetes as well, which is a common risk factor of liver cancer. B. Sub-point: One of the other medical uses of coffee is in preventing Alzheimer’s disease and dementia. 1. Supporting details: The most common neurodegenerative disease and number 1 cause of dementia is Alzheimer’s. 2. There is no cure for Alzheimer’s, however it has been shown that coffee drinkers have up to a 65% lower risk of getting the disease. [You might be thinking coffee is a miracle drink after some of those statistics, however it also has many negative effects on health as well. ] III. Main Point: There are a great deal of health issues that can result from drinking coffee, with some of the most common being addiction, insomnia and increased blood pressure. A. Sub-point: As a college student, one of the last things you will ever want to experience is insomnia, but it is a regular side effect of coffee. 1. Supporting details: By blocking certain receptors in the brain, coffee prevents chemicals that induce sleep from being used. B. Sub-point: Another widely studied effect of coffee, specifically caffeine, is how addictive it is. 1. Supporting details: Caffeine stimulates the central nervous  system and regular use will result in a physical dependence. 2. If a regular coffee drinker doesn’t have their daily cup, they will feel fatigued, irritable, and experience headaches within the first 24 hours. Conclusion 1. Transition Signal: In conclusion, coffee isn’t for everyone but if you do choose to consume it, you at least now know how it affects you. 2. Summary of Main Points: Throughout this speech we looked at how coffee first gained popularity in the human race, some of it’s positive health benefits as well as some of the negatives. 3. Call to Action: Even if you’ve never been a fan of coffee, try drinking a cup  before your next study session and see if you still don’t like it. 4. Memorable end: Although it may be addicting and cause serious potential health problems, there must be something special about coffee considering over half of adults in the U. S. drink it daily. References: 1) â€Å"Coffee Acts Just like Cocaine, Says Scientist. â€Å" BeverageDaily. com. Beverage Daily, 02 Sept. 2002. Web. 12 Nov. 2014. 2) Goldschein, Eric. â€Å"11 Incredible Facts About The Global Coffee Industry. † Business Insider. Business Insider, Inc, 14 Nov. 2011. Web. 12 Nov. 2014. 3) Gunnars, Kris. â€Å"13 Proven Health Benefits of Coffee† Authority Nutrition. N. p. , n. d. Web. 12 Nov. 2014. 4) â€Å"The History Of Coffee. † – National Coffee Association. NCA, n. d. Web. 12 Nov. 2014. 5) Stromberg, Joseph. â€Å"This Is How Your Brain Becomes Addicted to Caffeine. † Smithsonian. N. p. , 9 Aug. 2013. Web. 11 Nov. 2014. 6) Van Dam, Rob. â€Å"Ask the Expert: Coffee and Health. † The Nutrition Source. Harvard School of Public Health, n. d. Web. 09 Nov. 2014. 7) Weber, Belinda. â€Å"Coffee Consumption Cuts Liver Cancer Risk. † Medical News Today. MediLexicon International, 22 Oct. 2013. Web. 12 Nov. 2014. .

William Shakespeare A Brief Biography - 651 Words

William Shakespeare is arguably the most well known and successful author is the history of literature. Little is known about Shakespeare’s childhood and is what questions he’s existence. Besides the lack of knowledge of his childhood, Shakespeare lived a successful adult life. His plays changed the english language language forever. In all of his success, people still doubt he ever existed. William Shakespeare’s birth is unknown but church records show that he was baptised on April 26, 1564. It is guessed that if his parents followed tradition, he was born three days before his baptism on April 23. William was the son of John Shakespeare and Mary Arden who lived in the town of Stratford upon Avon. Stratford upon avon was a small country town at the intersection of a country road and the river Avon. John Shakespeare was a successful leather merchant up until the birth of his four sons and two daughters. Public records show that John’s fortunes soon began t o decline and eventually he became a public official. This would be key later down the road for William. No indication of where William went to school is one reason why people question his existence. It is believed though that William went to a school for public officials children only called The King’s New School. If he did attend here, William would have learned reading, writing, and the classics. Many scholars doubt that William attended here because he would have had to learn elsewhere in order to get the knowledgeShow MoreRelatedBrief Biography of William Shakespeare1433 Words   |  6 PagesThe Life of William Shakespeare William Shakespeare was a very dynamic playwright and writer. He enjoyed history which was shown through his writing. He excelled at writing many kinds of different genres of plays. He especially enjoyed tragedy and history. 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The Theory Of Leadership Self Assessment - 1355 Words

Leadership Self-Assessment As you go through your nursing career, you will find that many different theories can apply to everyday situations you encounter. I feel that even though I have been a nurse for 3  ½ years, I am still growing, learning, and taking in all kinds of new information and experiences from other nurses. The two theories of leadership I will evaluate myself on in this paper will be the skills approach and the team leadership theory. The skills approach shifts our focus from personality characteristics to an emphasis on skills and the abilities that can be learned and developed. It also suggests that knowledge and abilities are needed to have effective leadership (Northouse, 2016 p. 43). Due to changing my nursing setting from med/surg to home health, I don’t feel confident enough to be a leader quite yet. I do however, feel as I learn I can lead others with my experiences. 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